Methods for providing cytoplasmic male sterile Petroselinum crispum plants, cytoplasmic male sterile Petroselinum crispum plants and seeds and plant parts thereof
Glas, Cornelis,Dol, Nicolaas Johannes,Van Cappellen, Witte,Schrijver, Albertus Johannes Maria
申请号：
AU2012395191
公开号：
AU2012395191A1
申请日:
2012.11.22
申请国别(地区):
AU
年份:
2015
代理人:
摘要：
The present invention relates to methods for providing cytoplasmic male sterile Petroselinum plants, cytoplasmic male sterile Petroselinum crispum plants and to seeds, cells, tissues and plant parts thereof. Specifically the present invention relates to a method for providing a cytoplasmic male sterile Petroselinum crispum plant comprising: (a) providing first protoplasts obtained from a plant selected from the group consisting of Daucus carota L., Foeniculum vulgare Mill., Apium graveolens L., and Pastinaca sativa L., wherein the first protoplasts have a substantially inactivated nuclear genome and a substantially non-modified cytoplasm (b) providing second protoplasts obtained from Petroselinum crispum wherein the second protoplasts have a substantially inactivated cytoplasm and a substantially non-modified nuclear genome (c) fusing said first and second protoplasts and (d) obtaining from the fusion product of said first and second protoplasts a cytoplasmic male sterile Petroselinum crispum plant..
