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ANIMALS RESISTANT TO THE PIG REPRODUCTIVE RESPIRATORY SYNDROME VIRUS
专利权人:
ДЗЕ КЬЮРЕЙТОРЗ ОФ ДЗЕ ЮНИВЕРСИТИ ОФ МИССУРИ (US)
发明人:
ПРАТЕР Рэндалл С. (US)
申请号:
RU2013155677/10
公开号:
RU2013155677A
申请日:
2012.05.16
申请国别(地区):
RU
年份:
2015
代理人:
摘要:
1. A genetically modified pig resistant to infection by the pig reproductive and respiratory syndrome virus (VRRS), in which at least one SIGLEC1 gene allele has been inactivated. A genetically modified pig resistant to swine reproductive and respiratory syndrome virus (VRRS) virus infection, in which at least one CD163 gene allele is inactivated, wherein inactivation of the CD163 allele results in a CD163 protein that is unable to bind and / or expose the reproductive-respiratory syndrome virus pigs (VRRS). 3. A genetically modified pig according to claim 1 or 2, characterized in that both alleles of the SIGLEC1 gene were inactivated. A genetically modified pig according to claim 1 or 2, characterized in that both alleles of the CD163 gene were inactivated, moreover, inactivation of the CD163 alleles leads to the CD163 protein, which is unable to bind and / or expose the pig reproductive-respiratory syndrome virus (BPPCC) .5. A genetically modified pig resistant to swine reproductive and respiratory syndrome virus (VRRS) virus infection, in which at least one SIGLEC1 gene allele was inactivated and at least one CD163 gene allele was inactivated. 6. A method for producing a genetically modified pig resistant to swine reproductive and respiratory syndrome virus (VRRS) virus infection, in which at least one allele of the SIGLEC1 gene has been inactivated, comprising: enucleation of a pig oocyte; fusion of an oocyte with a fibroblast cell of a donor pig containing in the genome fibroblast cells at least one inactivated SIGLEC1 allele; oocyte activation to produce an embryo. 7. The method of claim 6, wherein1. Генетически модифицированная свинья, резистентная к инфекции вирусом репродуктивно-респираторного синдрома свиней (ВРРСС), у которой, по меньшей мере, один аллель гена SIGLEC1 был инактивирован.2. Генетически модифицированная свинья, резистентная к инфекции вирусом репродуктивно-респираторного синдрома свиней (ВРРСС), у которой инактивирован, по меньшей мере, один
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