A mite propagation inhibiting sheet has a sheet layer, an adhesive layer, and an unwoven fabric layer wherein mites are allowed to invade from a surface of the unwoven fabric layer to which an adhesive is not adhered, thereby forming a colony and the mites are trapped by an adhesive of the adhesive layer when the mites invade deep in the unwoven fabric layer by instinct, and further, a method of manufacturing a mite propagation inhibiting sheet, and a method of inhibiting the propagation of mites.