THE RESEARCH FOUNDATION FOR MICROBIAL DISEASES OF OSAKA UNIVERSITY
发明人:
MIYATAKE, HIROSHI,宫武浩,宮武浩
申请号:
TW106108834
公开号:
TW201738257A
申请日:
2017.03.17
申请国别(地区):
TW
年份:
2017
代理人:
摘要:
The present invention relates to a purification method for target protein, using a layered double hydroxide represented by general formula (I) below. General formula (I): [M<;SP>;2+<;/SP>; 1-x M<;SP>;3+<;/SP>; x(OH)2][An- x/n.mH2O] (In general formula (I) above: M<;SP>;2+<;/SP>; and M<;SP>;3+<;/SP>; are a divalent metal and a trivalent metal, respectively; A<;SP>;n-<;/SP>; is an interlayer anion; x is given by [M<;SP>;3+<;/SP>;]/([M<;SP>;2+<;/SP>;]+[M<;SP>;3+<;/SP>;]; and x is 0.1-0.9, n is 1-3, and m is 0-100).本發明係關於使用以下之通式(I)所表示之層狀雙氫氧化物(Layered Double Hydroxide)而將標的蛋白質純化之方法。通式(I):[M2+1-xM3+x(OH)2][An-x/n.mH2O](於上述通式(I)中,M2+與M3+分別為二價與三價之金屬,An-為層間陰離子。x係以[M3+]/([M2+]+[M3+])表示,x為0.1~0.9,n為1~3,m為0~100)