INSERM (Institut National de la Santé et de la Recherche Médicale);Université Paris 7 - Denis Diderot
发明人:
Catherine Le Visage,Didier Letourneur
申请号：
US15347595
公开号：
US20170080123A1
申请日:
2016.11.09
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The present invention relates to a method for preparing a porous scaffold for tissue engineering. It is another object of the present invention to provide a porous scaffold obtainable by the method as above described, and its use for tissue engineering, cell culture and cell delivery. The method of the invention comprises the steps consisting of: a) preparing an alkaline aqueous solution comprising an amount of at least one polysaccharide, an amount of a cross-linking agent and an amount of a porogen agent b) transforming the solution into a hydrogel by placing said solution at a temperature from about 4° C. to about 80° C. for a sufficient time to allow the cross-linking of said amount of polysaccharide and c) submerging said hydrogel into an aqueous solution d) washing the porous scaffold obtained at step c).
