Non-therapeutic use of collagen hydrolyzate to stimulate the biosynthesis of extracellular matrix proteins by skin cells, in order to increase the tonicity of the skin, reduce wrinkle formation and / or increase the moisture content of the skin. skin, characterized in that the collagen hydrolyzate is prepared by the successive action of two endoproteases with a different specificity, at a temperature of 50 to 60 ° C, for 120 to 180 min, using as a first enzyme a Bacillus subtilis endoprotease or Bacillus amyloliquefaciens and as a second enzyme a Bacillus licheniformis endoprotease, because at least 45% by weight of the collagen hydrolyzate has a molecular weight of less than 1,500 Da, and because the collagen hydrolyzate comprises at least four characteristic peptides with a molecular weight between 600 and 1,200 Da which, in a molecular weight distribution determined by mass spectroscopy MA LDI, have an intensity at least double compared to their environment, the mass spectrum having been determined using a TOF / TOF mass spectrometer with a sample of the collagen hydrolyzate which, adjusted in 0.1% trifluoroacetic acid at a final concentration 10 μg / μl, it has been purified using μC18 material and prepared with an HCCA matrix on a MALDI target.Utilización no terapéutica de hidrolizado de colágeno para estimular la biosíntesis de proteínas de matriz extracelular por células de la piel, con el fin de aumentar la tonicidad de la piel, de reducir la formación de arrugas y/o de aumentar el contenido en humedad de la piel, caracterizada por que el hidrolizado de colágeno está preparado mediante la acción sucesiva de dos endoproteasas con una especificidad diferente, a una temperatura de 50 a 60 °C, durante 120 a 180 min, utilizándose como primera enzima una endoproteasa de Bacillus subtilis o de Bacillus amyloliquefaciens y como segunda enzima una endoproteasa de Bacillus licheniformis, por que al menos un 45% en peso del hidrolizado de colágeno presenta un peso mole
