The present invention provides compositions and methods for regulating expression of heterologous nucleotide sequences in a plant. Compositions include two novel promoter nucleotide sequences for the genes encoding gamma tonoplast intrinsic protein and plasma membrane intrinsic protein in soybean, as well as vectors, microorganisms, plants and plant cells comprising the promoter nucleotide sequences, or variants and fragments thereof. Methods for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein are also provided. The methods comprise stably incorporating into the genome of a plant cell a nucleotide sequence operably linked to the promoter of the present invention and regenerating a stably transformed plant that expresses the nucleotide sequence.
