PROBLEM TO BE SOLVED: To provide a technique to duplicate and translocate any large region in Aspergillus chromosome across a wide range, so that it will be possible to and stably and systematically acquire an Aspergillus strain having a new trait, which was un-acquirable by conventional techniques.SOLUTION: A fungus belonging to genus Aspergillus wherein a transformation marker gene with deficiency of a terminal part at the 5 end or 3 end of its coding region is integrated into an outside of a target region in a chromosome of the fungus subject to duplicated translocation, and a transformation marker gene with deficiency of a terminal part at the 3 end or 5 end is integrated into an outside of a region in another chromosome of the fungus to be replaced with the target region.COPYRIGHT: (C)2014,JPO&INPIT【課題】麹菌の染色体上の任意の大領域を広範囲に亘って重複転座させる技術を提供し、従来は取得不能であった新たな形質を持つ麹菌の取得を安定的・計画的に可能にすること。【解決手段】或る染色体上の重複転座の対象となるターゲット領域の外側にコード領域の5’ 末端又は3’ 末端の一部が欠損した形質転換マーカー遺伝子が組み込まれ、該ターゲット領域と置き換えられる別の染色体上の領域の外側には、3’ 末端又は5’ 末端の一部が欠損した該形質転換マーカー遺伝子が組み込まれていることを特徴とするアスペルギルス属に属する菌。【選択図】図1
