The purpose of the present invention is to provide a method for producing frozen mesenchymal cells that maintain the same viability and adhesion rate as cells that have undergone a culture step, without going through a step for culturing the cells. The present invention provides a method for producing frozen mesenchymal cells that maintain the same viability and adhesion rate as cells that have undergone a culture step, without going through a step for culturing the cells, by treating tissue by a proteolytic enzyme, dispersing the cells, and cryopreserving the cells.
