The present invention relates to a method for producing CRM197 recombinant protein in cells. The method comprises culturing a cell comprising an expression plasmid with a polynucleotide and inducing expression of the CRM197 protein.本發明關於在細胞中生產CRM197重組蛋白質之方法。該方法包括培養包含具有多核苷酸的表現質體之細胞,並誘導該CRM197蛋白質之表現。