A tissue closing device includes an elongate arrangement device of which a distalend portion can penetrate an opening (e.g. wound hole) penetrating an livingtissue membrane and which has a handling portion on the proximal side, and a clipwhich is detachably retained at a distal end portion of the arrangement deviceand which closes the opening. The arrangement device includes a retaining member(e.g. thread) for detachably retaining the clip and pulling the clip in the proximaldirection, a cover tube, and a fixed tube. The handling portion has a first elasticmember (e.g. coil spring). When a restriction for maintaining the coil springin a deformed state (i.e. active state) is canceled, the thread is moved to theproximal direction by the restoring force of the coil spring in the conditionwhere the clip is locked to a distal end portion of the fixed tube, and the clip ispulled in the axial direction by the thread, whereby a deformation portion isdeformed.
