The present invention relates to the use of a herbicide-tolerant protein,wherein the method forcontrolling weeds comprises applying a herbicide containing an effective doseof tribenuron-methylto a plant growth environment where at least one transgenic plant is present,wherein the transgenicplant comprises a nucleotide sequence encoding a thifensulfuron hydrolase inits genome, andcompared to other plants without the nucleotide sequence encoding thehydrolase, the transgenicplant has reduced plant damage and/or an increased plant yield. The presentinvention discloses forthe first time that a thifensulfuron hydrolase can show a high tolerance to atribenuron-methylherbicide, plants containing a nucleotide sequence encoding the thifensulfuronhydrolase are stronglytolerant to the tribenuron-methyl herbicide and can at least tolerate 1-foldfield concentration, andthus the hydrolase has broad application prospects in plants.
