PROBLEM TO BE SOLVED: To provide a novel technology for introducing foreign mitochondria (exogenous mitochondria) genetically different from mitochondria (endogenous mitochondria), which are endogenous to cells, into the cells.SOLUTION: The present invention provides a method for introducing mitochondria into cells, the method comprising: a procedure of applying an alternating current electric field in which the alternating current electric field is applied to a mixture solution of GUV provided with a negative surface potential and cells to arrange the GUV and the cells； and a procedure of applying a pulsed direct current electric field in which the pulsed direct current electric field to the mixture solution to at least partially fuse the membrane of the GUV and the membrane of the cells.SELECTED DRAWING: Figure 1【課題】細胞に内在するミトコンドリア（内因性ミトコンドリア）とは遺伝的に異なる外来のミトコンドリア（外因性ミトコンドリア）を細胞内に導入するための新たな技術の提供。【解決手段】ミトコンドリアが封入され、負の表面電位を付されたＧＵＶと、細胞と、の混合液に交流電場を印加して、前記ＧＵＶと前記細胞とを配列させる交流電場印加手順と、前記混合液に直流パルス電場を印加して、前記ＧＵＶの膜と前記細胞の膜とを少なくとも一部融合させる直流電場印加手順と、含む、細胞内へのミトコンドリア導入方法を提供する。【選択図】図１
