The present invention relates to a preheated sterilizing box for medical laboratory equipment, comprising a body, a cover hinged to the body through a hinge, and an attachment device for preventing collision between preheated equipment. The cover comprises a cover housing, a UV lamp disposed on the cover and a transparent cover board. The body is separated by the separator and the heat guiding plate into a first chamber for holding preheated sterilizing equipment, a second chamber disposed with temperature control circuitry, a third chamber disposed with a heating material and a fourth chamber disposed with a battery. An insulating layer is disposed on the inside wall of the first chamber and the third chamber, a temperature sensor is embedded in the insulating layer disposed on the inside wall of the first chamber. A number of heat guiding poles and through holes are disposed on the heat guiding plate. The attachment device is disposed on the through holes. The sterilizing box has a sensible structure, having the advantages of simple, convenient to use, well portable, can effectively solve the problem of secondary contamination during preheating and sterilizing of medical laboratory equipment.