KNAP, Inge,KNARREBORG, Ane,LESER, Thomas Dyrmann,LUND, Bente,CANTOR, Mette Dines,DERKX, Patrick
申请号：
ES09795409
公开号：
ES2613805T3
申请日:
2009.12.16
申请国别(地区):
ES
年份:
2017
代理人:
摘要：
Composition of Bacillus subtilis, comprising 105 to 1012 CFU / g of Bacillus subtilis spore cells, where the composition of Bacillus subtilis is characterized by the fact that: (i): Bacillus subtilis spores have germination and growth Rapids of spore to vegetative cell in the presence of a bile salt medium comprising 4 mM of bile salts and in the presence of a bile salt medium comprising 6 mM of bile salts, defined by the fact that the spores of Bacillus subtilis reach a vegetative cell growth point of 0.4 OD630 within 18 and 19 hours, respectively, where the vegetative cell growth point is the point on the growth curve where the OD value begins to increase (due to growth of vegetative cells) in a certain continuous sense and reaches an OD630 of 0.4； (I): where the bile salt medium is the non-selective Calf Infusion Broth (VIB) medium of Example 1 herein supplemented with a bile salt mixture comprising the taurodeoxylate of conjugated bile salts and glycodeoxycholate and the deoxycholate bile salt deconjugated in the proportions 60% of taurodeoxycholate, 30% of glycodeoxycholate and 10% of deoxycholate； and (II): where the OD test analysis is carried out by the following steps: (a): fill a well in a microtiter plate with 0.150 ml medium of bile salt with 108 Bacillus subtilis spores per average ml (ie , this is zero time)； and (b): incubate the plate at 37 ° C under atmospheric conditions and measure the OD630 values, using a spectrophotometer and with stirring before each reading, to obtain a representative growth curve over time； and (ii) Bacillus subtilis vegetative cells produce an enzyme or an amino acid selected from the group consisting of phenylalanine, valine, threonine, tryptophan, isoleucine, methionine, leucine, lysine, cysteine, tyrosine, histidine and arginine； and with the proviso that the vegetative cells of Bacillus subtilis are not cells, as described in claim 1 of PCT / EP2008 / 057296 and the priority application thereof, EP07111 939.0, published
