An insecticidal and ovicidal composition and an insecticidal and ovicidal method by using thereof, which composition comprises: (a) triacetate glycerol 50-99.9 part of weight, which contains more than 50 % oleic acid for forming lipid acid, (b) nonionic surfactant 0.1-20 part of weight and (c) glycerol derivatives 0-30 part of weight. The composition of the present invention has not only insecticidal effects but also ovicidal effects for agricultural pests, further has safety and high insecticidal and ovicidal effecs.一種除蟲除卵組成物及使用此組成物的除蟲除卵方法,該除蟲除卵組成物係包含:(a)三酸甘油酯50至99.9質量份,其含有油酸50%以上以作為構成脂肪酸;(b)非離子性界面活性劑0.1至20質量份;以及(c)甘油衍生物0至30質量份。本發明之組成物對於農作物害蟲不僅擁有除蟲性,亦具備除卵性,係具有既安全且高的除蟲除卵效果。