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Cell line for screening influenza virus polymerase assembly inhibitor and construction method thereof
专利权人:
Suzhou Institute of Systems Medicine
发明人:
XiaoFeng Qin,Chunfeng Li,Zining Wang,Taijiao Jiang
申请号:
US15737826
公开号:
US10526634B2
申请日:
2016.12.28
申请国别(地区):
US
年份:
2020
代理人:
摘要:
Provided are a cell line PA/PB1 HEK293T (Teton) for screening an influenza virus polymerase assembly inhibitors and a construction method thereof. The PA/PB1 HEK293T (Teton) cell line is constructed by integrating a vector expressing PA-GlucC fusion protein and a vector expressing GlucN-PB1 fusion protein into a HEK293T (Teton) cell via a triple plasmid system of pMDLg/pRRE, pRSV-Rev and pMD2.G. The HEK293T (Teton) cell line is formed by transforming a TOP10 competent cell with a synthesized Teton-3G gene fragment ligated to a lentiviral vector FG.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/
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