Cell line for screening influenza virus polymerase assembly inhibitor and construction method thereof

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专利权人:

Suzhou Institute of Systems Medicine

发明人:

XiaoFeng Qin,Chunfeng Li,Zining Wang,Taijiao Jiang

申请号：

US15737826

公开号：

US10526634B2

申请日:

2016.12.28

申请国别(地区):

US

年份:

2020

代理人:

摘要：

Provided are a cell line PA/PB1 HEK293T (Teton) for screening an influenza virus polymerase assembly inhibitors and a construction method thereof. The PA/PB1 HEK293T (Teton) cell line is constructed by integrating a vector expressing PA-GlucC fusion protein and a vector expressing GlucN-PB1 fusion protein into a HEK293T (Teton) cell via a triple plasmid system of pMDLg/pRRE, pRSV-Rev and pMD2.G. The HEK293T (Teton) cell line is formed by transforming a TOP10 competent cell with a synthesized Teton-3G gene fragment ligated to a lentiviral vector FG.

来源网站：

中国工程科技知识中心

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http://www.ckcest.cn/home/