Hypoxia environment simply and high sensitivity fluorescence the chemical compound which is displayed with below-mentioned formula (i) in order to measure (as for1 R - CO-N (R11) - Y-N1 (R12) - X-1 (X2)m -64 p-CH-N=N-Ar-R13 [as for11 R and12 R H or alkyl (the alkylene which is joined mutually is included), as for1 Y alkylene, as for1 X single bond, - CO-, or - SO-2, as for2 X -2 O-Y-N (R14) - (as for2 Y as for alkylene and14 R H or alkyl), as for m 0 or 1,64 as for p-CH-ha p-hueniren and Ar as for arirujiiru and13 R to show the alkylamino], as for2 R alkyl and the alkoxykarubokishi, sulfo, or alkoxy carbonyl R3 - As for8 R H or halogen).低酸素環境を簡便かつ高感度に蛍光測定するための下記の式(I)で表される化合物〔R1は-CO-N(R11)-Y1-N(R12)-X1-(X2)m-p-C6H4-N=N-Ar-R13 [R11及びR12はH又はアルキル(互いに結合したアルキレンを含む)、Y1はアルキレン、X1は単結合、-CO-、又は-SO2-、X2は-O-Y2-N(R14)-(Y2はアルキレン、R14はH又はアルキル)、mは0又は1、p-C6H4-はp-フェニレン、Arはアリールジイル、R13はアルキルアミノ]を示し、R2はアルキル、アルコキシ、カルボキシ、スルホ、又はアルコキシカルボニル;R3~R8はH又はハロゲン〕。
