An objective of the present invention is to provide a production method of a sheet for regenerating a cartilage tissue, which uses a conventional sheet-shaped porous body comprising a biological absorbency synthetic high polymer, such as polylactic acid, polyglyc-olic acid and a copolymer of lactic acid and glycolic acid. The sheet for regenerating the cartilage tissue can differentiate chondrocytes or stem cells without culturing the cells by pressurizing such as a pressurizing culture like the conventional method, and can accumulate the cells in a supporting carrier with high efficiency. The sheet for regenerating the cartilage tissue is produced by the steps of; seeding chondrocytes or stem cells differentiating to the chondrocytes on a sheet-shaped porous body comprising a biological absorbency synthetic high polymer; taking the porous body into a culture liquid; applying acceleration of 100 to 1000G to the porous body for a predetermined time; and culturing the porous body without applying the acceleration.
