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TRANSGENIC PLANT PRODUCTION METHOD FOR PRODUCING GENETICALLY MODIFIED PLANT AND GENETICALLY MODIFIED TRANSGENIC PLANT PRODUCED BY SAID PRODUCTION METHOD
专利权人:
SUMITOMO RUBBER INDUSTRIES; LTD.
发明人:
Chiharu TANI,Akari OKADA,Yukino INOUE
申请号:
US15335223
公开号:
US20170145430A1
申请日:
2016.10.26
申请国别(地区):
US
年份:
2017
代理人:
摘要:
Provided is a transgenic plant production method for producing a genetically modified rubber-producing plant that is capable of efficient short-term gene transfer, of introducing a target gene while retaining the useful traits of a plant undergoing gene transfer, and of efficient redifferentiation. The method includes: a culture step of culturing a tissue fragment from a rubber-producing plant to obtain a cultured tissue fragment; a transformation step of transforming, with a gene construct, the cultured tissue fragment obtained in the culture step; and a regeneration step of regenerating a plant from the tissue fragment transformed in the transformation step, wherein the transformation step includes: an infection step of culturing the cultured tissue fragment in the presence of an Agrobacterium transformed with a gene construct; and a decontamination step of culturing the cultured tissue fragment obtained in the infection step in a decontamination medium containing a carbapenem antibiotic to decontaminate the Agrobacterium.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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