Provided is a manufacturing method of a microneedle array capable of closing a needle-like recessed portion with a drug solution and providing a high yield.A manufacturing method of a microneedle array includes: an adjustment step of positioning and adjusting a mold having a first surface and a second surface, which oppose each other, and a plurality of needle-like recessed portions in the first surface, and an ejection nozzle which ejects a drug solution in a first direction； a relative movement step of moving the mold and the ejection nozzle relative to each other to cause a position of the needle-like recessed portion and a position of the ejection nozzle to coincide with each other in a plan view in the first direction； an ejection step of ejecting the drug solution from the ejection nozzle toward the needle-like recessed portion； a vibration step of vibrating the mold to move the drug solution toward the needle-like recessed portion and close the needle-like recessed portion with the drug solution after the ejection step； and a suction step of suctioning the second surface of the mold.
