A physiological signal collection unit is for use with a physiological signal measuring apparatus to measure physiological signals from an anatomical part of a test subject, in which the anatomical part is covered with hair. The physiological signal collection unit includes a detector and a wearable member. The detector includes a circuit substrate defining a substrate-through-hole to permit hair strands to extend through, an electrical connector, and at least one signal collecting component. The wearable member is to be worn on the anatomical part of the test subject, and is formed with a through-hole having a size smaller than size of the circuit substrate. The through-hole permits the hair strands extending through the circuit substrate to extend through the wearable member.
