Vincent C. Bond,Michael POWELL,Ming Bo HUANG,Syed ALI
申请号：
US15660572
公开号：
US20170369533A1
申请日:
2017.07.26
申请国别(地区):
US
年份:
2017
代理人:
摘要：
The present application relates to methods of producing exosomes. The application also provides a method for preparing a protein composition comprising culturing an exosome-producing cell expressing a Nef-fusion protein comprising a Nef-derived peptide fused to a protein of interest； isolating exosomes from the exosome-producing cell culture； and purifying the protein of interest from the isolated exosomes. The application further discloses compositions that comprise exosomes containing the Nef-fusion protein, as well as methods of using the Nef-fusion protein and exosomes containing the Nef-fusion protein.
