DONGGUK UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION;동국대학교 산학협력단
发明人:
IM, Gun Il,임군일,LEE, Jong Min,이종민,KO, Ji Yun,고지윤
申请号：
KRKR2017/006328
公开号：
WO2018/030630A1
申请日:
2017.06.16
申请国别(地区):
KR
年份:
2018
代理人:
摘要：
The present invention relates to a nonviral minicircle vector expressing a SOX gene, a stem cell having the vector introduced thereinto, a pharmaceutical composition comprising the stem cell for prevention or treatment of diseases of the cartilage, and a method for constructing the vector. The transformation of mesenchymal stem cells with MC/SOX-Trio or MC/SOX-Duo, which are both nonviral minicircle vectors according to the present invention, can completely exclude the necessity of expensive growth factors that have been indispensably used in inducing the differentiation of mesenchymal stem cells into chondrocytes. Hence, the mesenchymal stem cells transformed therewith, when implanted in vivo, can differentiate into chondrocytes by themselves, having the advantage of simplifying the conventional complicated steps of culturing cells to induce differentiation and then transplanting the cells. Further, in contrast to conventional vector systems wherein antibiotic-resistant genes and other bacteria-derived exogenous genes are simultaneously introduced into cells even after transformation, the vector of the present invention is designed to regulate two or three SOX genes necessary only for chondrogenic differentiation under one promoter, with the minimum introduction of unnecessary genes into target cells and can, therefore, be utilized as a nonviral vector system in the most advantageous form for use in clinical application of stem cell-gene therapeutic agents.La présente invention concerne un vecteur non viral en minicercle exprimant un gène SOX, une cellule souche présentant le vecteur introduit en son sein, une composition pharmaceutique comprenant la cellule souche pour la prévention ou le traitement de maladies du cartilage et un procédé de construction du vecteur. La transformation de cellules souches mésenchymateuses avec MC/SOX-Trio ou avec MC/SOX-Duo, qui sont tous les deux des vecteurs non viraux en minicercle selon la présente invention, peut exclure complèt
