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On-slide staining by primer extension
专利权人:
The Board of Trustees of the Leland Stanford Junior University
发明人:
Samusik, Nikolay,Nolan, Garry P.,Goltsev, Yury,McIlwain, David Robert
申请号:
AU2015280326
公开号:
AU2015280326A1
申请日:
2015.06.19
申请国别(地区):
AU
年份:
2017
代理人:
摘要:
A method for analyzing planar sample is provided. In some cases the method comprises: (a) labelling the planar sample with a capture agent that is linked to a nucleic acid, wherein the capture agent specifically binds to complementary sites in the planar sample (b) reading a fluorescent signal caused by extension of a primer that is hybridized to the nucleic acid, using fluorescence microscopy. Several implementations of the method, and multiplexed versions of the same, are also provided.
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