LIU JANG-RYOL;JEONG WON-JOONG;MIN SUNG-RAN;JBONG SEOK-WON;HAN SU-KYOUNG
发明人:
LIU JANG-RYOL,JEONG WON-JOONG,MIN SUNG-RAN,JBONG SEOK-WON,HAN SU-KYOUNG
申请号：
US20050500664
公开号：
US2005204433(A1)
申请日:
2005.05.20
申请国别(地区):
美国
年份:
2005
代理人:
摘要：
The objective of this invention is to enhance the efficiency of plastid transformation using nuclear transformed plants in which the microbial recombinase A(recA) is to target to (or expressed in) the plastid. This invention will be better explained by the following detailed descriptions. A plant is transformed with a nuclear transformation vector containing the microbial recA gene added with a plastid targeting sequence. In this nuclear transformed plant, the frequency of plastid transformation is enhanced greater than two-folds due to increased homologous recombination between the plastid transformation vector carrying genes of interest (or target genes) and the plastid genome. In addition, because plastid transformation is accomplished through a gradual process, adventitious shoots selected after being subjected to plastid transformation should be cut into explants, and then shoots regenerated from the explants are to be reselected until all of the plastids in the shoots are uniformly transformed. However, when the nuclear transformed plant is used, the number of reselection is reduced to ?? to 1/3 due to increased homologous recombination.
