The invention relates to a preparation method of a novel recombinant antibiotic polypeptide medicine, and relates to a production technology of recombinant antibiotic medicine.The steps are as follows: (1) preparing E.coli strains containing recombinant plasmids.Cryopreservation,(2) using liquid production medium to expand the culture of bacteria.(3) inducing expression of recombinant antimicrobial peptide.Isolation and purification of recombinant antibacterial peptide；It is characterized byThe formulation of the liquid production medium is as follows: the mass volume ratio of each component in the final solution is 0.4% - 0.7% disodium hydrogen phosphate.Potassium dihydrogen phosphate 0.1% - 0.6%Ammonium chloride 0.05% - 0.2%,Calcium chloride 0.0005% - 0.001%,Magnesium sulfate 0.5% - 2.5%,Protein 1% - 3%,Yeast powder 0.5% - 1%Glucose 0.1% - 0.5%,The rest is water.The invention further optimizes the method in terms of expanding the culture parameters, which is suitable for large-scale production of antibacterial peptides and the prepared antibacterial peptides have high purity.本發明係為一種新型重組抗菌多肽藥物的製備方法，涉及重組抗生素藥物生產技術。包括如下步驟：(1)製備含有重組質粒的大腸桿菌菌種，冷凍保存，(2)採用液體生產培養基中對菌種進行擴大培養，(3)誘導表達重組抗菌多肽，分離純化重組抗菌多肽；其特徵在於，所述液體生產培養基的配方如下：各組分在終溶液中的質量體積比為：磷酸氫二鈉0.4%-0.7%，磷酸二氫鉀0.1%-0.6%，氯化銨0.05%-0.2%，氯化鈣0.0005%-0.001%，硫酸鎂0.5%-2.5%，蛋白腖1%-3%，酵母粉0.5%-1%，葡萄糖0.1%-0.5%，氯化鈉0.2%--0.8%其餘為水。本發明還進一步在擴大培養參數方面對該方法進行了優化，適合於大規模生產抗菌肽且其製備得到的抗菌多肽純度高。
