Church, George M.,Mali, Prashant G.,Esvelt, Kevin M.
申请号：
AU2020203977
公开号：
AU2020203977A1
申请日:
2020.06.15
申请国别(地区):
AU
年份:
2020
代理人:
摘要：
#$%^&*AU2020203977A120200702.pdf#####ABSTRACT Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign 5 nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the 10 DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.1/65 mENNIMMI, .mMEN .mMEN mMEME .mMEMEN lmmml Ixxx : i xxx : i loomm ....... ........ ImMEMEM ....... ........ ISERE........ .. IN mossm, mEMMUNI, lmmm Mllxxx : i z E IMEN lmmm Mllxxx : i xx ImEMEME, cn d .-.1 SM ................ ................. ................. .................. .................. .................... ..................... ....................... .................. ................ ................ ............... ................ ................ ................ ............... ................ ................ L L ................ ............... ................ ................ ................ ............... ................ ................ ................ ............... ................ ................ ................ ............... ...... ..... ...... .... ...... ..... ...... .... ...... ...... ....... ..... ....... ..... ....... .... ...... ...... ...... ..... ...... ....... ...... ...... ...... ..... ...... ...... ...... .....
