PROBLEM TO BE SOLVED: To provide a method of generating cells which secrete neurotrophic factors.SOLUTION: Cells which secrete neurotrophic factors are generated by incubating a population of undifferentiated mesenchymal stem cells (MSCs) in a differentiating medium comprising basic fibroblast growth factor (bFGF), platelet derived growth factor (PDGF), heregulin and cAMP.SELECTED DRAWING: Figure 25【課題】神経栄養因子を分泌する細胞を作製する方法の提供。【解決手段】未分化間葉系幹細胞(MSC)の集団を、塩基性線維芽細胞増殖因子(bFGF)、血小板由来増殖因子(PDGF)、ヘレグリン、及び、cAMPを含む分化用培地においてインキュベーションし、それにより神経栄養因子を分泌する細胞を作製する。【選択図】図25