DR NAGARAJAN SELVAMURUGAN,MS S DHIVYA,DR MARIAPPANADAR VAIRAMANI
申请号：
IN201841000270
公开号：
IN201841000270A
申请日:
2018.01.03
申请国别(地区):
IN
年份:
2018
代理人:
摘要：
Abstract Title: Chitosan, Calcium Polyphosphate and Pigeonite for Bone Tissue Repair Treatment of critical-sized bone defects with cells and biomaterials offers an efficient alternative to traditional bone grafts. Chitosan (CS) is a natural biopolymer that acts as a scaffold in bone tissue engineering (BTE). Polyphosphate (PolyP), recently identified as an inorganic polymer, acts as a potential bone morphogenetic material, whereas pigeonite (Pg) is a novel iron-containing ceramic. In this study, we prepared and characterized scaffolds containing CS, calcium polyphosphate (CaPP), and Pg particles for bone formation in vitro and in vivo. CS/CaPP scaffolds and CS/CaPP scaffolds containing varied concentrations of Pg particles (0.25%, 0.5%, 0.75%, and 1%) were prepared and characterized by SEM, XRD, EDAX, FT-IR, degradation, protein adsorption, mechanical strength, and biomineralization studies. The cytocompatibility of these scaffolds with mouse mesenchymal stem cells (mMSCs, C3H10T1/2) was determined by MTT assay and fluorescence staining. Cell proliferation on scaffolds was assessed using MUSE„ cell analyzer. The effect of scaffolds on osteoblast differentiation at the cellular level was evaluated by Alizarin red (AR) and alkaline phosphatase (ALP) staining. At the molecular level, the expression of osteoblast differentiation marker genes such as Runt-related transcription factor-2 (Runx2), ALP, type I collagen-l (Col-I), and osteocalcin (OC) was determined by real time reverse transcriptase (RT-PCR) analysis. Bone regeneration was assessed by X-ray radiographs, SEM and EDAX . analyses, and histological staining such as hematoxylin and eosin staining and Massons trichrome staining (MTS) in a rat critical-sized tibial defect model system. The inclusion of iron-containing Pg particles at 0.25% concentration in CS/CaPP scaffolds showed enhanced bioactivity by protein adsorption and biomineralization, compared to that shown by CS/CaPP scaffolds alone. Incr
