Mononuclear cell proliferation agent, mononuclear cell proliferation culture medium, the manufacturing method of monocyte, the manufacturing method of the manufacturing method of Dendritic Cells and dendritic cell vaccine
The present invention provides the means that can efficiently and easily make mononuclear cell proliferation. The present invention provides a kind of mononuclear cell proliferation agent used before the differentiation processing from monocyte to Dendritic Cells, contain one or more of Flt-3L, IL-3 or IFN-γ. In addition the present invention also provides the mononuclear cell proliferation culture medium used before the differentiation processing from monocyte to Dendritic Cells, contain one or more of Flt-3L, IL-3 or IFN-γ. Mononuclear cell proliferation culture medium of the invention can also contain GM-CSF.本發明提供能高效且簡便地使單核細胞增殖的手段。本發明提供了一種在從單核細胞到樹突狀細胞的分化處理前使用的單核細胞增殖劑，其含有Flt‑3L、IL‑3或IFN‑γ中的一種以上。另外本發明還提供了在從單核細胞到樹突狀細胞的分化處理前使用的單核細胞增殖用培養基，其含有Flt‑3L、IL‑3或IFN‑γ中的一種以上。本發明的單核細胞增殖用培養基也可含有GM‑CSF。
