Methods are provided for isolating and using a whole- saliva leech extract. The methods can include feeding a phagostimulatory agent to a leech； inducing a regurgitation in the leech , the inducing including placing the leech in an environment having a temperature of less than about 0°C； and , collecting an unrefined , whole saliva in the regurgitation of the cooled leech. The methods can include revitalizing the leech by warming it at a temperature ranging from about 5°C to about 40°C. Stable , lyophilized , whole -saliva extracts of a leech are also provided , the extract having a stable activity when stored for use at a temperature below about -20°C, the extract maintaining at least 70% of the activity for at least 6 months. The extracts can be used to treat solid tumors, treat liquid tumors , treat diabetes, treat a viral disease, treat a parasitic disease , treat an antibacterial disease , or serve as an anti -oxidant.
