The present invention relates to a method to eliminate microorganisms and a composite therefor. The method is used to eliminate microorganisms by mixing fruit juice from at least one, selected from among a calamansi, a lemon, and a lime, with essential oil selected from among carvacrol and thymol. As such, the present invention is capable of effectively controlling microorganisms as well as being safely applied to food products.COPYRIGHT KIPO 2017본 발명은 깔라만시, 레몬 및 라임에서 선택되는 하나 이상의 과일로부터의 과일즙 및 카바크롤 및 티몰에서 선택되는 하나 이상의 에센셜오일을 혼합처리하여 미생물을 사멸시키는 방법 및 이를 위한 조성물에 관한 것이다.