INDIANA UNIVERSITY RESEARCH & TECHNOLOGY CORPORATION
发明人:
JOHNSON, Raymond, M.
申请号：
USUS2013/059529
公开号：
WO2014/043397A1
申请日:
2013.09.12
申请国别(地区):
US
年份:
2014
代理人:
摘要：
Two patients diagnosed with KLS were treated. One patient had severe KLS that progressed to the equivalent of pediatric Kawasaki Disease Shock Syndrome (KDSS). The second patient had a typical KLS presentation and clinical course. Cytokines and chemokines provide inflammatory signatures in the serum that reflect the polarity of the immune response and the affected cell types. Multiplex ELISA technology was used to define the cytokine milieu in the serum of the two adult HIV patients with KLS during the acute and convalescent phases. Those sera were compared with sera from asymptomatic HIV subjects and a normal serum control. Those comparisons suggest that HIV KLS is a dysfunctional Th2 response to an unknown inciting agent in the vascular wall, and that a multiplex ELISA or similar technology based a limited combination of KLS/KD pathogenesis-related cytokines (IL-6, IL-13, sTNFRII) and endothelial/smooth muscle chemokines (CCL1, CCL2, CxCL11) may provide an objective tool for diagnosing KLS and Kawasaki Disease. Because KD and HIV KLS are the only known "Th2" vasculitidies that spare the lungs (unique clinical presentation) and include plasma cell infiltration of the vascular wall as a prominent histopathologic feature (unique pathophysiology), a diagnostic test based on combinations of the above analytes will be highly specific and therefore clinically useful.Selon l'invention, deux patients souffrant de KLS diagnostiqué ont été traités. Un patient avait une KLS sévère qui progressait vers l'équivalent du syndrome du choc de la maladie de Kawasaki (KDSS) pédiatrique. Le second patient présentait une KLS et une évolution clinique typiques. Les cytokines et les chimiokines fournissent des signatures inflammatoires dans le sérum qui montrent la polarité de la réponse immunitaire et les types cellulaires touchés. Une technologie ELISA multiplexe a été utilisée pour définir le milieu de cytokines dans le sérum des deux patients adultes, atteints par le VIH, souffrant d
