To provide a sperm collection device which can be repeatedly used without a hygienic problem because a core member can be easily rinsed while maintaining a merit of accommodating the core member in a vessel and can arbitrarily control a stimulus applied to the core member by hand while a rubbing operation is performed, the sperm collection device comprises an open/close unit 2 composed of two open/close members 4 openably and closeably supported about one end and core pieces 10 opened and closed by being supported by the open/close members, respectively forming an insertion empty space between the closed core pieces, and a holder 30 for detachably supporting the open/close unit in a closed state.