КОЙЛ Энтони (US),БАКА Мануэль (US),ТИСТЕД Томас (US),ДРАБИК Стейси (US),ГРИНБЕРГ Люба (US),НОВАРРА Шабазз (US),ОГАНЕСЯН Вахех (US),ХЕРБСТ Роналд (US),СПЕНСЕР Дэвид Кеннет (US)
申请号：
RU2014117511/10
公开号：
RU2014117511A
申请日:
2012.10.10
申请国别(地区):
RU
年份:
2015
代理人:
摘要：
1. Tn3-frame structure containing a CD40L-specific monomeric subunit, where the monomeric subunit contains seven beta chains designated A, B, C, D, E, F and G, and six loop sections designated AB, BC, CD, DE, EF and FG, and where this Tn3-frame structure specifically binds to CD40L.2. The Tn3 frame structure according to claim 1, containing one CD40L-specific monomeric subunit. The Tn3-frame structure according to claim 1, containing two CD40L-specific monomeric subunits joined in tandem. The Tn3 framework structure of claim 3, wherein the two CD40L-specific monomeric subunits are connected directly. The Tn3 framework structure of claim 3, wherein the two CD40L-specific monomeric subunits are linked by a linker. The Tn3 framework structure of claim 5, wherein the linker comprises a peptide linker. The Tn3 framework structure of claim 6, wherein the peptide linker is a flexible peptide linker. The Tn3 framework structure of claim 7, wherein the peptide linker comprises the sequence (GX), where (a) X is serine (S), alanine (A), glycine (G), leucine (L), isoleucine (I), or valine (V)； (b) m and n are integers； (c) m is 1, 2, 3 or 4； and (d) n is 1, 2, 3, 4, 5, 6, or 7.9. The Tn3 framework structure of claim 8, wherein the peptide linker comprises SEQ ID NO: 131, SEQ ID NO: 132, SEQ ID NO: 142, or SEQ ID NO: 143.10. The Tn3 frame structure according to claim 3, wherein the binding of the frame structure to CD40L is improved compared to the Tn3 frame structure containing one CD40L-specific monomeric subunit. The Tn3 frame structure according to claim 3, wherein binding of the frame structure to CD40L improves the effect on the target compared to the Tn3 frame structure containing one CD40L-specific monomeric subunit. Tn3 frame structure according to claim 10, where the improvement of binding1. Tn3-каркасная структура, содержащая CD40L-специфичную мономерную субъединицу, где мономерная субъединица содержит семь бета-цепей, обозначенных А, В, С, D, Е, F и G, и шесть петлев
