It is intended to provide a method of producing athree-dimensional culture of chondrocytes comprisingculturing the chondrocytes under culture conditions inwhich the chondrocytes are embedded in a gelled-structurecomprising collagen and proteoglycan. Also provided is amethod of chondrocyte production comprising chondrocytes ina gelled structure, a gelled structure for regenerating acartilage tissue, a kit comprising the gelled structure andan artificial transplantation material for generatinginjured cartilage.