A medical file and first aid assembly for storing medical files along with first aid supplies includes a storage case that is carried by a trainer of a sports team. The storage case has a plurality of trays that is pivotally positioned therein for storing first aid supplies. A file accordion is movably coupled to the storage case for storing medical history of each player on the sports team. In this way the trainer has access to medical history of each player for safely applying first aid to each player. A communication unit is coupled to the storage case and the communication unit is in electrical communication with an extrinsic communication network thereby facilitating inventory of the first aid supplies to be tracked and remotely ordered for re-supply.