The present invention provides vaccination methods using microneedle arrays which, when applied to the skin of a subject, create a defined total pore volume. Arrays may be chosen of varying total microneedle volumes (small, intermediate or large) for use in immunisation regimes to induce different T-cell and antibody responses in the subject. The present invention also provides kits for use in such vaccination method(s) comprising the appropriate microneedle array(s).
