KIM, SANG HEON,김상헌,KIM, SOO HYUN,김수현,JUNG, YOUNG MEE,정영미,PARK, IN SU,박인수
申请号：
KR1020110071672
公开号：
KR1020130010796A
申请日:
2011.07.19
申请国别(地区):
KR
年份:
2013
代理人:
摘要：
PURPOSE: A method for differentiating stem cells into smooth muscle cells and a smooth muscle cell-scaffold complex for tissue engineering are provided to effectively differentiate human adipose-derived stem cells into the smooth muscle cells.CONSTITUTION: A method for differentiating stem cells into smooth muscle cells comprises a step of applying stress to a stem cell-inoculating elastic scaffold in a medium containing biochemical differentiation factor of TFG-beta-1, platelet-derived growth factor-BB(PDGF-BB), ascorbic acid, retinoic acid, or angiotensin II. The biochemical differentiation factor is retinoic acid. The elastic scaffold is formed of biodegradable polymers. The biodegradable polymer is a copolymer of lactic acid and carprolactone. A smooth muscle cell-scaffold complex for tissue engineering contains the differentiated smooth muscle cells for vascular disease treatment or wound healing.COPYRIGHT KIPO 2013본 발명은 줄기세포가 접종된 탄성 스캐폴드에 응력(Strain)을 가하면서 줄기세포를 평활근세포로 분화시키는 방법 및 상기 방법에 의해 분화된 평활근세포를 함유하는 혈관 질환 또는 창상 치료를 위한 조직공학용 평활근세포 패치에 관한 것이다. 본 발명은 기계적 응력과 생화학 인자의 인간 지방줄기세포의 평활근세포 분화에 미치는 시너지 효과를 증명하였고 기계-활성 조직 공학에 있어서 줄기 세포의 유용한 응용법을 제공한다.
