An isolated anti-SCUBE2 antibody or a binding fragment thereof is disclosed. The anti-SCUBE2 antibody comprises an antigen binding region that specifically binds to a target domain located within SCUBE2 (SEQ ID NO: 66) and exhibits a property of inhibiting VEGF-induced angiogenesis. The target domain is selected from the group consisting of the EGF-like motifs 4 to 6 ranging from a.a. position 175 to 323, or the spacer region ranging from a.a. position 441 to 659, or the first cys-rich motif ranging from a.a. position 668 to 725 of SCUBE2 (SEQ ID NO: 66). The anti-SCUBE2 antibody or binding fragment thereof is for use in treating a disease associated with VEGF-induced angiogenesis, or for use in treating a tumor or inhibiting tumor angiogenesis and cancer cell growth in a subject in need thereof.本發明揭示分離之SCUBE2抗體或其結合片段。該SCUBE2抗體包含一個抗原結合區域，能專一性的結合於SCUBE2 (SEQ ID NO：66)之標靶域且有能力抑制VEGF誘導之血管新生。該標靶域選自以下結構區域：SCUBE2 (SEQ ID NO：66)之EGF-like結構模組(EGF-like motifs)4至6 (a.a.175至323)、間隔區域 (spacer region) (a.a.441至659)，或第一個富含半胱胺酸結構模組 (cys-rich motif) (a.a.668至725)。該SCUBE2抗體或其結合片段可應用於治療在有需要的個體中與VEGF誘發血管新生之相關疾病，例如藉由遏止腫瘤血管新生而達成降低癌細胞生長之目的。
