The present invention proposes a magnetic nanocomposite with multi-biofunctional groups, which comprises a core and a shell wrapping the core, wherein the core contains magnetic nanoparticles, and wherein the shell is made of a conductive polymer with multi-biofunctional groups where a medicine, an antibody or a fluorescent label can be attached.本發明提供一種具有多重反應性官能基之磁性奈米粒子複合物及其製備方法,其包含有一核層;以及一包覆於核層外的殼層,其中核層係由磁性奈米粒子所組成,殼層是具有多重反應性官能基之導電高分子所構成,藉此可作為藥物載體或固定上螢光偵測物子或特殊抗體。10‧‧‧核層12‧‧‧殼層