An object information acquisition apparatus according to an embodiment of the present invention includes a plurality of transducer elements each configured to transmit an acoustic wave to an object, to receive reflected waves reflected from inside the object, and to convert the reflected waves into a time-series reception signal; and a processor configured to perform frequency domain interferometry combined with adaptive signal processing by using the reception signals output from the plurality of transducer elements and a reference signal so as to obtain acoustic characteristics at a plurality of positions located inside the object. The processor is configured to switch the reference signal to another reference signal at least once in accordance with a target position located inside the object while performing the frequency domain interferometry so as to obtain the acoustic characteristics at the plurality of positions located inside the object.