INSTITUTE OF ANIMAL SCIENCE AND VETERINARY MEDICINE SHANDONG ACADEMY OF AGRICULTURAL SCIENCES
发明人:
JUN LI,SHUO WANG,XIAOYAN WU,JIANLI SHI,ZHE PENG,CHEN LI,SHAOJIAN XU
申请号：
NL2025015
公开号：
NL2025015A
申请日:
2020.02.28
申请国别(地区):
NL
年份:
2020
代理人:
摘要：
The present invention provides a method for efficiently expressing a PCV2 Cap and PCV3 Cap fusion protein. First, a recombinant baculovirus that efficiently expresses the PCV2 Cap protein and the PCV3 Cap protein is constructed: the nucleotide sequence of 5 the PCV2 Cap protein with its nuclear localization signal truncated is linked to the nucleotide sequence of the PCV3 Cap protein through a hydrophobic flexible protein linker sequence, and a honeybee melittin signal peptide is added to the N-terminal of the PCV2 Cap protein sequence and the PCV3 Cap protein sequence to promote their secretion and expression. The proteins expressed by sf9 insect cells infected with the 10 recombinant positive baculovirus have unden/vent a variety of post-translational modifications, are close to natural virus-encoded proteins, and have high biological activity. Moreover, the baculovirus is highly species-specific, only infects Insect cells and are non-infectious to vertebrate cells, and their expression products are safe and reliable and can be used in subsequent experiments after simple processing. Therefore, 15 for expression of PCV2 Cap and PCV3 Cap fusion proteins, the method of the present invention is safer and more effective than traditional methods.
