Yang Xiao,Jinlong Cui,Zhengrong Li,Qing Ye,Lingyun He,Xuegen Wang
申请号：
US15572845
公开号：
US20180133154A1
申请日:
2015.07.08
申请国别(地区):
US
年份:
2018
代理人:
摘要：
A preparation method of a Decoy nucleic acid cationic liposome carrier including: (1) mixing dioleoyl phosphoethanolamine and (2,3-dioleoyl-propyl)-trimethylamine in a mass ratio of 4:1 to 1:4, and adding an organic solvent to obtain a mixed solution through dissolution (2) completely evaporating the organic solvent in the mixed solution obtained in step (1), dissolving the remaining solid fraction by using an HEPES buffer solution, firstly hydrating the solution for 30 to 60 min, and then ultrasonically processing the solution for 30 to 60 min (3) filtering the mixed system obtained after processing in the step (2) by a membrane of 0.4 to 0.8 μm, then filtering the mixed system by a membrane of 0.03 to 0.2 μm, and preparing uniformly to distributed blank liposome with small grain size and (4) mixing the blank liposome with protamine and Decoy nucleic acid according to a mass ratio of (50 to 120):(10 to 20):1, and incubating for 12 to 24 h at the temperature of 2° C. to 8° C. to form the complete Decoy nucleic acid cationic liposome carrier.
