Disclosed is a method of fractionation of sulfated glycan molecules derived from xylan using organic solvent where the steps include: (a) dissolving unfractionated sulfated glycan molecules in solution； (b) adjusting the pH of the solution to make an alkaline solution； (c) performing at least one fractionation cycle by adding organic solvent to the alkaline solution. Also disclosed are the use of the molecules prepared by this method in the manufacturing a medicament for treating non-infective inflammatory diseases, ischaemis, cancer, viral disease, interstitial cystitis, Crohn&rsquo；s disease, ulcerative colitis, Reiter&rsquo；s syndrome, a haematoma, a haemorrhoid, frost bite or a burn.
