The present invention relates to a biological sample preservation tube with an identification code, wherein an information element which records the sample information is fused integrally with the bottom wall and/or side walls during molding the preservation tube, the preservation tube is suitable for the preservation of a biological sample at low temperature and can ensure the effectiveness and stability at low temperature and at the same time also relates to a method for manufacturing the biological sample preservation tube, wherein the preservation tube is molded by a one-step process, without an additional secondary processes for manufacturing the identification code, the method improves the production efficiency, reduces the production cost, employs multi-point symmetrical feeding, and has good quality and stability of the product, so that the method is of great significance as it opens up a new idea for developing and producing the similar product in the industry, especially the small-sized preservation tube with an identification code.