PURPOSE: A prefabricated box and a bio-retention structure using the same are provided to reduce costs by easily producing, transporting, and constructing the bio-retention structure. CONSTITUTION: A prefabricated box(100) comprises a plurality of columns, a side plate, and a lower plate. The side plate is detachably attached to the side part between the columns. The lower plate is detachably attached to the lower part between the columns. A bio-retention structure comprises a constructed wetland box and a vegetation penetration ditch box. The side and lower plates are attached to or detached from a frame composed of a plurality of columns as necessary to form the constructed wetland box or vegetation penetration ditch box.본 발명은 복수의 기둥(110); 복수의 기둥(110) 사이 측부에 착탈가능하게 설치되는 측면판(200); 복수의 기둥(110) 사이 하부에 착탈가능하게 설치되는 저면판(300);을 포함하는 바이오리텐션용 조립식 박스(100)를 제시함으로써, 제작, 운반, 시공이 간편하고, 비용을 줄일 수 있으며, 다양한 구조의 구현이 가능하고, 유지관리가 용이하도록 한다.