The present invention relates to a composition comprising (i) a first substance which is capable to stimulate T cells, (ii) a second substance which is capable to stimulate NK cells (natural killer cells), and (iii) lipopolysaccharide (LPS) and wherein the second substance is a double stranded nucleic acid, single stranded nucleic acid, unmethylated CpG oligodeoxynucleotide, TLR agonist except lipopolysaccharide (LPS), arabinoxylan (BioBran® MGN-3), an immunoglobulin, a murine cytomegalovirus (MCMV)-encoded protein, CCL5 (chemokine (C-C motif) ligand 5), a UL-16-binding protein (ULBP), CD48, CD70, CD155, CD112, Necl-1, B7-H6, ICAM-1, RAE-1 (retinoic acid early inducible 1), H60, Multl and/or hemagglutinin, to a method for measuring, determining and/or detecting the status of cell-mediated immune responsiveness of a subject, to a kit comprising the composition according to the invention, to the use of the composition for measuring, determining and/or detecting of cell-mediated immunity (CMI) and/or for detecting, diagnosing, monitoring an immunosuppression condition in a subject.La présente invention concerne une composition comprenant (i) une première substance capable de stimuler les lymphocytes T, (ii) une seconde substance capable de stimuler des cellules NK (cellules tueuses naturelles), et (iii) un lipopolysaccharide (LPS) la seconde substance étant un acide nucléique à double brin, un acide nucléique à simple brin, un oligodésoxynucléotide CpG non méthylé, un agoniste de TLR à lexception dun lipopolysaccharide (LPS), un arabinoxylane (BioBran® MGN-3), une immunoglobuline, une protéine codant un cytomégalovirus murin (MCMV), un CCL5 (ligand de chimiokine (motif C-C) 5), une protéine de liaison UL-16 (ULBP), CD48, CD70, CD155, CD112, Necl-1, B7-H6, ICAM-1, RAE-1 (acide rétinoïque inductible précoce 1), H60, Multl et/ou hémagglutinine un procédé permettant de mesurer, déterminer et/ou détecter létat de la réactivité immunitaire à médiation cellulaire dun sujet
