An herbal formulation employing a plurality of ingredients extracted from a plurality of herbs for the regeneration of cardiomyocytes by reducing the after effects of myocardial infarction is disclosed. the herbal formulation made from one or all of the ingredients include Terminalia arjuna, Pashanbhedi (Bergenia lingulata), Jatamansi (Nardostachys jatamansi), Swarnabhasma (gold ultra fine powder), Gandhakrasayan, Trifala (Myrabilis), Commiphoramukul, Triphala (three myrobalans) andcalcined pearl shell powder (Mukthapisthi) in appropriate percentage by weight of the composition. According to the method of preparing the herbal formulation, the ingredients are purified by liquid solvent extraction and homogenization of herbal ingredients is achieved to attain a homogenous formulation. The formulation is prepared in any of the forms include but not limited to a gelatin capsule, a vegetarian capsule, a tablet, a liquid, a syrup, a dairy beverage and a snack bar.